Analysis Software Features

OptiView offers several data processing tasks and quantitative analysis functions for TD files generated from time-resolved fluorescence experiments. The options available for bioluminescence and continuous wave experiments are limited to viewing intensity image maps and applying the OptiView Image Tools to those intensity images. OptiView is pre-installed on all system computers, but can also be installed “off-line” on your own computer as an independent tool for image and data analysis.

OptiView is simple to learn and easy to use, and offers unique workspace saving tools that allow you resume an analysis at any time and to share your findings, task customization tools to streamline repetitive operations, and sophisticated software algorithms that can remove autofluorescence for improved signal-to-noise and signal-to-background ratios and superior detection.

For analysis purposes, the following are available at different workflow steps for scans originating from time-resolved fluorescence experiments:

  • Detailed scan information from acquisition TD files, including laser power, integration time, scan time, and excitation wavelength.
  • Tracking of correction parameters applied to scan data and parameters selected for data processing and post-processing tasks.
  • The camera and profile views of the specimen.
  • Display and comparison of TPSFs from acquisition files, with scaling for photon counts and normalized counts.
  • TPSF fits using a one- or two-exponential fitting model. Lifetime, amplitude, and fitting accuracy are also available after the fit.
  • Distance (spatial) measurements.
  • Intensity measurements and computation of the average, standard deviation, and number of points included in the selection.
  • Computation of depth and concentration from a single scan point.
  • Image export to BMP, JPG, and TIFF file formats, as well as to TXT, CSV, and DICOM file formats.
  • Reports formatted in TXT files.
  • A range of tasks that allow you to remove background fluorescence from scan data and generate data sets of fluorescence lifetime and depth and concentration data.

OptiView Tasks

Tasks are actions you can perform on processed or post-processed data. In general, tasks provide a method to prepare data for further processing or for analysis. Some tasks, such as the automatic data correction that optimizes acquired raw data, are initiated automatically whenever a TD file generated from a fluorescence experiment is opened. Others, such as generating lifetime analysis data, are available on demand. Custom tasks, which can include modified default tasks and other custom tasks, can also be created to optimize an analysis workflow.

Each available task includes basic parameters with preset default values, as well as advanced parameters for more experienced users. You can save edited parameters as the application default settings or create a custom task with those parameters. Tasks can also be configured to run automatically after the “File Open” operation is complete.

Remove Background. This task allows you to remove background fluorescence from loaded scan data. Scan data with background removed can be defined as net fluorescence data.

Lifetime Analysis. Lifetime and lifetime fitting image maps, as well as histograms and weighted histograms, are available for lifetime analysis data. For multi-lifetime fits, additional lifetime maps are available to display the short and long lifetimes contained in the data and the short and long lifetime contribution.

Depth-Concentration Analysis. This task allows you to quantify the depth and qualify the concentration of a selected scan region. Depth and concentration maps, as well as topography images showing fluorophore concentration and location combined with the 3D specimen profile, are available for depth-concentration analysis data. Slices of concentration represented in depth are also available.