Analysis Software – 3D Reconstruction
The optional 3D Reconstruction module enhances the functionality of OptiView by providing a means to generate and visualize volumetric data from time-resolved measurements as:
- Volume slices, in which each slice corresponds to an X, Y or Z-axis position. Viewing volume slices can help you to obtain a more complete view of the reconstruction.
- A 3D Plane viewer that includes an interactive 3D re-slicing box that allows you to view all axis representations simultaneously.
- Concentration volume isosurfaces within the specimen profile.
Volume Slices. A volume can also be shown as slices in a Slice viewer, shown below, in which each slice corresponds to an X, Y, or Z-axis position. Volume slices can help you to obtain a more complete view of the reconstructed data by allowing you to view depth localization and the spread of the reconstructed fluorescent region. As a reference, the contour of the profile is displayed for each slice.
Volume Planes. Generated volumes can also be shown in a Plane viewer that includes an interactive 3D re-slicing box that displays all axis representations simultaneously. This view can provide a detailed overview of the reconstructed fluorophore localization and provides plane translation and rotation.
Concentration Volume Isosurfaces. Isosurfaces allow you to view the shape of the reconstructed fluorophore region. An isosurface is fully described by a threshold — which is often called the iso-value — an opacity value, and the respective data to threshold. The threshold determines the object boundary and binarily partitions the visualized volume into two regions. Voxels with a data value equal to the threshold and voxels between areas with data values lower than the iso-value form the isosurface. Isosurfaces can be used to display tissue boundaries, as shown below.
