What Customers Are Saying
“The development of the Optix time-domain imaging system takes an important step forward in whole-body small animal fluorescence imaging techniques to advance it from a qualitative tool to a volumetric representation of the fluorophore concentration distribution.”
“In building our world class In Vivo Imaging Shared Facility, we wanted to have a time-domain based fluorescence imaging system as an important part of our comprehensive collection of instrumentation for macro-scale molecular imaging. We plan to leverage Optix’s powerful combination of unmatched sensitivity in the detection of quantum dots, as well as the system’s capabilities for fluorescence lifetime gating for multiplexing signals, 3D reconstruction, and co-registration with microCT.”
“We selected an optical imaging system that acquired data in the time domain. This mode of acquisition offers the ability to decouple the tissue attenuation measured by simpler continuous wave mode into the underlying tissue absorption and scattering. The consequence is a more detailed description of photon propagation, which leads to a more accurate recovery of probe concentration.”
“To clearly differentiate between probe-derived specific fluorescence signals and autofluorescence, the property of the Optix system to measure fluorescence lifetime is of great advantage.”
“Time-domain technology allows for whole-body NIR fluorescence lifetime analysis, based on both the specificity of fluorescence probes and the sensitivity of their emission lifetime to environmental characteristics. The tumor’s metabolic microenvironment can vary significantly within a tumor and between tumors of the same grading and clinical staging.”
“The time-domain mode of acquisition, based on time-correlated single-photon counting, enables more accurate recovery of probe concentration. Moreover, fluorescence lifetime-based imaging can discriminate exogenous near-infrared probes from autofluorescence based on their photophysical decay constants, even when their fluorescence intensities are comparable.”
“Time-domain offers the opportunity to measure the fluorescence lifetime of a probe. This permits the simultaneous measurement of multiple probes, which are distinguishable by lifetime, or to use probes that have a lifetime-environment sensitivity to yield additional information about the environment.”
“Dye-mediated fluorescent lifetime imaging is an exciting optical imaging method with a great potential to unravel physiological and molecular processes in cells and living organisms.”