Neuroscience
Courtesy of Abedelnasser Abulrob
National Research Council of Canada, Institute for Biological Sciences
Ottawa, Canada
Imaging brain-targeted FC5 immunoliposomes
Delivering therapeutics to the brain can be a pharmaceutical challenge due to the presence of the blood brain barrier. This study demonstrates that FC5-targeted liposomal drug carriers could be exploited as an integrated brain imaging and therapeutic delivery platform and provides proof of concept for an optical imaging ‘surrogate’ to assess brain delivery of targeted therapeutic formulations.
Top row: Fluorescence intensity images.
Bottom row: Volume concentration planes.
Paper
The blood-brain barrier transmigrating single domain antibody: mechanisms of transport and antigenic epitopes in human brain endothelial cells
Abedelnasser Abulrob, Hein Sprong, Paul Van Bergen en Henegouwen, and Danica Stanimirovic. Journal of Neurochemistry, Volume 95, Number 4 / November 2005: pp. 1201-1214.
Courtesy of Abedelnasser Abulrob
National Research Council of Canada, Institute for Biological Sciences
Ottawa, Canada
Imaging brain-targeted FC5 immunopilosomes
Delivering therapeutics to the brain can be a pharmaceutical challenge due to the presence of the blood brain barrier. This study demonstrates that FC5-targeted liposomal drug carriers could be exploited as an integrated brain imaging and therapeutic delivery platform and provides proof of concept for an optical imaging ‘surrogate’ to assess brain delivery of targeted therapeutic formulations.
Top row: Fluorescence intensity images.
Bottom row: Volume concentration slices.
Paper
The blood-brain barrier transmigrating single domain antibody: mechanisms of transport and antigenic epitopes in human brain endothelial cells
Abedelnasser Abulrob, Hein Sprong, Paul Van Bergen en Henegouwen, and Danica Stanimirovic. Journal of Neurochemistry, Volume 95, Number 4 / November 2005: pp. 1201-1214.
Courtesy of Maxime Bouchard
Goodman Cancer Centre, McGill University
Montreal, Canada
Tumor localization to the cerebellum
Top row: Fluorescence intensity images of mouse brain in a GFP-Pax-2 transgenic animal and control. Both autofluorescence and GFP signals were detected in the transgenic animal (top left), while the control animal (top right) displayed autofluorescence only.
Bottom row: The same fluorescence intensity images as above gated between 2.5 and 3.0 ns.
The overall GFP signal in the mouse brain can be distinguished by a process known as gating. By isolating the lifetime signals that only fall within the range of 2.5-3.0 ns (GFP has a 2.7 ns lifetime), all autofluorescence (which has a lifetime of greater than 3.0 ns) is eliminated from the GFP-gated picture. When gating at 2.5-3.0 ns, the bottom right picture (control) has no GFP signal which can, however, be observed in the bottom left picture in the GFP transgenic mouse.
“The sensitivity of (time-domain optical imaging) in experimental settings surpasses that of small-animal MRI by several magnitudes, enabling detection of subtle changes in tracer concentrations.”
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